Double phase culture system mediated enhanced protocol for shoot proliferation of Vanilla andamanica Rolfe. - an endemic wild relative of commercial Vanilla from the Andaman and Nicobar Islands

A liquid overlay culture system in micropropagation improved shoot proliferation many species. In the present investigation, we have developed an enhanced protocol using double phase (DPS) for Vanilla andamanica, endemic species of Andaman and Nicobar Islands recognized under vulnerable category by IUCN. vitro generated nodal explants were used experiments tested Murashige Skoog medium augmented with various cytokinins (BAP, KIN, 2iP) auxins (IBA, NAA, IAA) to produce a maximum 3.24 ± 0.08 shoots per explant 5 µM BAP 5.23±0.67 roots basal MS medium. remarkable increase regeneration was observed when cultured on DPS optimum cytokinin (5 BAP). On evaluation influence conventional single-phase systems (SPS), (solid medium) exhibits multiplication rate optimal (5µM) average 8.66 0.17 explant, which represents 2-fold over SPS + BAP. The plantlets rooted at 100% frequency half-strength devoid acclimatized success. double-phase mediated proliferating procedure could be employed large scale commercial breeding trials better results regard V. andamanica.